Extraction Optimization and Characterization of Collagen from Yellow Pike Conger Swimbladder with Acid-Hydro-Exctraction Method
AbstractSwim bladder is one of marine potential byproducts for alternative source of collagen. This study aimed
to optimize hydro-extraction and characterized collagen. Extraction optimization of collagen was determined
using Box-behnken design response surface method with three variables: CH3COOH concentration, soaking
time and extraction time to yield response. Hydro-extraction collagen was characterization based on the
amino acid content, SDS-PAGE, FT-IR and DSC. Acording to the result, the concentration had significantly
influence yield. Optimum extraction conditions were variable combinations of acetic acid concentration of
0.1 M, for 1 h and hydro-extraction time of 1 h to produce the highest yield 63.35%. collagen was classified
as type I collagen by amino acid content, electrophoresis patterns and fourier transform infrared (FTIR)
spectra. The glass transition of collagen was 67.23oC, showed high thermal stability that can be applied to
the cosmetics industry and nutraceutical.
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